A simple precise reliable and sensitive isocratic stability indicating RP-UPLC assay method of Linezolid in tablets and for determination of content uniformity has been developed and validated for reducing analysis time and maintaining good efficiency, An isocratic separation of Linezolid was achieved on Waters Acquity BEH C18, 50 × 2.1 mm id, 1.7 μm particle size column with a flow rate of 0.25 ml/min and using photodiode array detector to monitor the elute at 245nm. Mobile phase consisting of methanol: water (50:50 v/v) to achieve good resolution and retention. The detector linearity was established by concentrations range of 1.5-80μg/ml (r2=0.999) with a limit of detection and quantification of 0.4 and 1.5 μg/ml respectively. Recovery of drug was achieved between 99 to 101%. The method was subjected to oxidation, hydrolysis, photolysis and thermal degradation. Stress studies did not interface with the detection of Linezolid and the assay in thus stability-indicating.
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